I'm having the exact same problem in Windows 7. Tried everything but nothing is working.
Turned off Windows Firewall.
Turned off Avast antivirus.
Port-forwarded every port from 50000 to 60000 the PC.
Watching Eye-Fi helper open close/fail ports in this range using Process Explorer TCP-IP monitor.
CAN NOT see why it is failing or how to get it working.
p.s. I have degree in computer science and 15 years experience working in I.T. - how is average Joe supposed to figure this out??? Why can't Eye-Fi tuning give more information instead of BS "check your internet connectivity and retry"
When I try and run Eye-Fi Center it immediately runs Firewall Tuning and then it fails at "Testing incoming connections" and then Eye-Fi Center shuts down. I paid big money for this 8Gb X2 card last November and I haven't been able to use it with my EOS-1D since buying it. Not recommending it to anyone I know
. Worst piece of software I've bought in a long time.